ABSTRACT
AIM: To optimize the extraction and purification of water-soluble active ingredient of Ferula sinkiangensis.METHODS: First,the comprehensive scoring system was set up to reflect the change of the extraction rate and ferulic acid coutent.in combination with the uniform design.RESULTS: The optimized extracting process was as follows: adding 8 times water and extracting twice,every time lasting 2.5 h.The optimized centrifugation purificating process consisted of: concentration ratio (1 ∶ 3),35 minutes centrifugal time,4 000 r/min rotation speed.The optimized ZTC1 + l-Ⅱ clarificant purification process was as follows: concentration ratio was 1 ∶ 22,the content of pacificator was 22% ,purification time was 1 h.CONCLUSION: The optimized extraction is available,reasonable,and reproducible.The results of pharmacodynamic test show that centrifugation is suitable to the purification of extract liquid.
ABSTRACT
AIM: To select the prescription of harmine hydrochloride cream and to study the anti-itching effect of harmine hydrochloride cream. METHODS: A comprehensive score system,including appearance,homogeneity,coating performance,viscosity,particle diameter,temperature-resistant test,and centrifugal effect were established as evaluative criteria. Then uniform design was used for selecting the prescription of harmine hydrochloride cream. The histamine phosphate and 5-hydroxytryptamine (5-HT) were used to induce mouse cutaneous pruritus. Then the anti-pruritic effect of harmine hydrochloride cream in contrast to Piyanping Ointment as control group. RESULTS: The optimized prescription was as follows: 8% of monostearin,5% of Vaseline,6. 1% of beeswax, 1. 1% of Span-60,1. 9% of Tween-80. The local application of harmine hydrochloride cream could alleviate the itching induced by histamine phosphate and 5-HT,respectively. And the anti-pruritic effect of harmine hydrochloride cream was stronger than the effect of the positive control group. CONCLUSION: The prescription of the harmine hydrochloride cream is available,reasonable,and reproducible. The harmine hydrochloride cream has the anti-pruritic effect.